Objective: To investigate the relationship between AMPK�±, PGC�±, insulin\nresistance and reproductive function in PCOS mice and to find out the molecular\nmechanisms and potential therapeutic molecular targets of pathogenesis\nof PCOS. Methods: The PCOS mouse model was established by DHEA administering\nwith Balb/c mice. And after AMPK agonist AICAR and inhibitor\nCompound C intervention, Fasting blood glucose, fasting insulin and testosterone\nlevels were observed. The HOMA index was calculated. The changes of\nPGC�± expression in ovarian tissue were observed by western blot and immunohistochemistry\nto determine the relationship between insulin resistance and\nPGC�± in PCOS mice. Results: Western blot and immunohistochemistry\nshowed that PGC1�± protein was expressed in the ovary of mice and the expression\nof PGC1�± was negatively correlated with AMPK�± in our study.\nCompared with the control group, the expression of PGC1�± in the ovaries of\nthe mice in PCOS group was significantly increased (P < 0.05), after intervention\nwith AMPK agonist AICAR, the expression of PGC1�± in PCOS + AICAR\ngroup was lower than that in PCOS group (P < 0.05). It is worth noting that\nthe expression of PGC1�± in PCOS mice exposed to AMPK inhibitor Compound\nC also decreased compared with PCOS group (P < 0.05). Conclusion:\nIn ovarian tissue, the insulin resistance-related AMPK�± pathway in PCOS\nmice may be negatively correlated with PGC�±.
Loading....